FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140397
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73860.6476691888877294No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG257.854171E-444.00259444
TGGTATC8300.035.1056142
GTGGTAT8850.034.1843071
GTCGCGT350.00410283931.42353227
GGTATCA22100.030.3651161
GTATCAA48400.029.9462641
TATCAAC55050.026.5645852
ATCAACG57100.025.6533833
TCAACGC57050.025.579524
CAACGCA57150.025.5358815
AGAGTAC54550.025.35451711
CAGAGTA55900.024.83839810
AACGCAG59700.024.7717516
CGCAGAG60500.024.2645788
ACGCAGA61750.023.9504187
GCAGAGT65250.022.5844359
AGTACGG61250.022.49421713
GAGTACG62700.022.0149812
TACGGGG27100.020.90621215
ACGGGGT6500.020.73290316
Produced by FastQC (version 0.11.5)