FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139359
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44170.3876741220282633No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT14160.12428040679013375No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGAC11860.10409361755162332No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8150.034.101861
TGGTATC8150.034.07942
GTATCAA41150.030.2261521
GGTATCA17850.027.903241
TATCAAC46500.026.7900472
TAGACCG550.001225052925.00523253
ATCAACG50400.024.546853
TCAACGC50500.024.4950164
CAACGCA51150.024.236425
AGAGTAC50100.023.9836211
CAGAGTA50800.023.759310
AACGCAG52500.023.6131976
CGCAGAG53300.023.2082238
ACGCAGA54300.022.8314427
GCAGAGT56800.021.829419
AGTACGG54800.021.57824113
TATACCG650.003248184721.1443425
ACGGGCG956.0559294E-520.26472716
TATAGGA956.0600232E-520.2629472
GAGTACG58950.020.01075712
Produced by FastQC (version 0.11.5)