FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411487
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24580.5973457241662555No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT6500.1579636780748845No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT5390.13098834228055806No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC4680.11373384821391683No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC4310.10474206961580802No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA150.005778503655.0059620
AAGACCG257.8699156E-443.978035
GCTAATG300.001918575436.688481
GTGGTAT3400.033.9908031
TTCGCAC350.00410663131.4128744
TGGTATC3600.031.3152222
GTATCAA19050.030.6218831
GGTATCA8150.028.0227991
CGTAGAC400.00786319427.5029813
ATCAACG23200.025.2383063
TATCAAC23450.025.2098222
AGAGTAC22450.025.1018711
AACGCAG23700.025.0507776
CAGAGTA22700.024.94651810
TCAACGC23550.024.8601914
CAACGCA24150.024.5839925
ACGCAGA24750.024.213077
CGCAGAG24600.024.025478
TAGGCCA701.8039865E-423.5596565
ACGGGGG2150.023.0285516
Produced by FastQC (version 0.11.5)