FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791323
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36090.45607166732168786No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31680.4003422117138008No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.2781417954488875No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT17310.21874759105952943No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA15350.19397894412269073No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14060.17767713057752652No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC13500.1706003743098583No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT12850.16238628221345772No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT11690.14772728708757357No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT11620.14684269255411508No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA10980.1387549711053514No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG10700.1352165929715173No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA10500.1326891800187787No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC10300.13016176706604005No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA10060.12712887152275368No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG8990.11360721222560194No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8700.1099424634441309No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA8110.1024865952335519No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT8070.10198111264300418No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA7930.10021192357608714No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG7920.1000855529284502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18850.051.628732
GTGGTAT19000.050.7962531
GTATCAA66850.039.8136861
TATCAAC76800.035.3042342
GGTATCA35850.034.274261
ATCAACG83400.032.612283
TCAACGC83650.032.3624084
AACGCAG85050.031.9795936
CAACGCA85500.031.8609875
GTCGAGG10150.030.9836778
TCGAGGT10300.029.7091359
ACGCAGA96400.028.3466177
CGCAGAG96450.028.3319238
CGTCAGA1554.451067E-927.417995
GCAGAGT100400.027.3883159
GGTAGCG957.449555E-526.8407698
GACAGGT3350.025.3830971
CAGAGTA50150.024.87321510-11
GTACTGG4300.024.7190061
CTAGACT1251.7505328E-523.7988154
Produced by FastQC (version 0.11.5)