FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791323
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT13580.17161133949095375No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT11880.1501283293926753No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC11420.14431527960137644No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11300.14279883182973324No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC10060.12712887152275368No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT9660.12207404561727637No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA9390.11866203813107921No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA9140.11550277194015593No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT9080.11474454805433432No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA8710.11006883409176785No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG8540.10792053308193998No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT8030.10147563005245645No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA7950.100464664871361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22100.040.1866231
TGGTATC22500.039.212912
GACTAGT453.7906007E-430.56089426
GTATCAA80300.025.8525091
AGAGTAC54050.024.98586511
CAGAGTA55700.024.34293210
CGGGATA2550.023.72963717
TATCAAC88700.023.3953672
GACAGGT1800.022.9134251
GCGGGAC600.002041892622.903293
GGTATCA41400.022.2492681
CGGGACC1155.0304334E-721.52550117
ATCAACG97300.021.2414473
AACGCAG97650.021.2202636
TCAACGC97300.021.1567084
TACTATA1307.032031E-821.1428322
CGGGTCA3150.020.95604317
GAGACGC1055.5058863E-620.95604311
CAACGCA98900.020.8700125
AGTACGG64100.020.63933213
Produced by FastQC (version 0.11.5)