FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198213
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30420.25387806675440844No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27990.2335978661556835No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18280.1525605213764164No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT16030.13378255785907847No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19450.043.9176332
GTGGTAT20850.041.393651
GTATCAA63500.038.0962561
TATCAAC73850.033.0311662
AACGCAG79850.030.8685046
ATCAACG80350.030.4119643
GTCGAGG11050.030.3840358
TCAACGC80500.030.3552954
CAACGCA81350.030.1426035
GGTATCA36050.028.8937951
ACGCAGA91900.026.7758757
CGCAGAG92800.026.3788058
TCGAGGT12550.025.3989819
GCAGAGT100200.024.4741049
AGCGTCA2700.023.6096743
GACAGGT6450.023.0702381
AGTCGAG17400.022.2265837
CAGAGTA56450.020.77991910-11
CGTCAGA2902.1827873E-1120.5159915
AGAGTAC54200.020.1526710-11
Produced by FastQC (version 0.11.5)