FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100432
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT82130.7463432542855897No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38710.35177094086685956No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28860.26226063945795836No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19860.1804745772569318No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT15080.1370370908879422Illumina Paired End PCR Primer 2 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT12350.11222865202029748Illumina Paired End PCR Primer 2 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12250.052.0504461
TGGTATC12650.050.393132
GTATCAA44800.040.040891
GGTATCA25150.037.0147971
TATCAAC49700.036.0849952
TCAACGC51800.034.293924
ATCAACG52250.034.07993
CAACGCA53000.033.6778225
AACGCAG55350.032.2494246
ACGCAGA59400.030.0492347
CGCAGAG60300.029.6712198
GCAGAGT66900.026.8710639
CAGAGTA39300.025.79089410-11
ATCGCGC850.001226823724.9989438
TATACGG900.001711439523.6101135
GAGTACG44350.022.94946712-13
AGAGTAC37650.021.89814610-11
GTACGGG46650.021.4986514-15
AGGGGGG21650.020.61394970-71
GTCCTAA1455.4973534E-520.5210381
Produced by FastQC (version 0.11.5)