FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100432
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166581.5137691379385552No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7850.038.2138671
TGGTATC8650.033.700622
GTATCAA36950.030.016131
TATCAAC40600.027.3655032
GGTATCA17300.025.9301851
ATCAACG42600.025.7532773
TCAACGC43050.025.4167424
CAACGCA43550.025.1892035
AGAGTAC40000.024.8861211
AACGCAG44800.024.3033756
CAGAGTA41450.024.27871710
CGGGATA2100.023.57333817
ACGCAGA46450.023.440077
CGCAGAG47100.023.1749618
GCAGAGT50750.021.454999
AGTACGG45600.021.34944213
GAGTACG47000.020.94660212
TACGGGA22450.019.723215
ACGGGAT7700.019.64444716
GTACGGG50850.019.03705814
Produced by FastQC (version 0.11.5)