FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359535
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6830.18996759703505917No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA5200.14463125982171415No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT3970.11042040413311639No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC3710.10318884114203068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGG300.001920732836.6788354
TGGTATC8750.032.0410042
TAACGGC1300.031.73687636
TAGTCCG350.004083698631.44775453
GTGGTAT9100.031.430241
GTCCGGA350.004097641431.42586755
AACGGCC1350.030.56143637
CTTGCGG453.792313E-430.55292755
CACATGT1450.030.34644128
CGCAAGG553.48297E-530.00159612
GGCCGCG1400.029.46995540
GCACCGC1400.029.46585310
TTTAACG1500.029.3308134
GGTACCC1350.028.52400846
GTGACAC1350.028.52003724
GCGGTAC1350.028.51209644
ACCGCCT1500.027.50146312
ACACATG1400.027.50146327
GTCTAAT805.1603456E-727.5014631
TGACACA1500.027.50146325
Produced by FastQC (version 0.11.5)