FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311066
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20830.15887834784823954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC24850.038.5972482
GTGGTAT25400.038.0068741
GTATCAA88250.025.5868741
TATCAAC96100.023.3644752
AGAGTAC68400.022.59708411
CAGAGTA70800.022.14184410
GGTATCA46500.022.0619871
ATCAACG102300.021.8930443
TCAACGC101800.021.8925974
AACGCAG103050.021.7603746
CAACGCA104600.021.3328325
TAACGGC956.0544113E-520.26577436
ACGCAGA113650.019.7564967
CGCAGAG114600.019.5484898
TGCCGAC856.6805E-419.4135355
TTGTACG1151.2187833E-519.116853
GCAGAGT119850.018.7896469
CTAACCG909.793071E-418.33990152
AGTACGG85450.017.73420513
GAGTACG86700.017.73229212
Produced by FastQC (version 0.11.5)