FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876834
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20550.23436591190578834No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.19376529650994373No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.1373121936421261No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.11906472604848808No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13300.042.8283121
TGGTATC13250.042.338632
GTATCAA44400.038.2003861
ATACCGT858.698389E-734.999186
TATCAAC50700.033.9489062
GGTATCA25100.033.36341
TCAACGC52100.033.1182254
ATCAACG52800.032.6791573
AACGCAG52550.032.672886
CAACGCA53750.032.1015745
GTCGAGG6400.029.2180638
ACGCAGA59550.028.6895037
CGCAGAG60800.028.0297688
GCAGAGT65650.026.023769
CGGGGTC4450.023.8758414
TCGAGGT7550.023.0789289
CAGAGTA39100.022.77120410-11
AGTCGAG10900.021.444457
TCGACAC1000.00315550121.24959
TACGGGG22950.021.1106152
Produced by FastQC (version 0.11.5)