FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382609
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT53190.3847074624857787No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38750.2802672339034391No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26850.19419807045954424No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA21730.1571666320702382No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT19940.14422009403960193No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA14920.10791192593133705No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT14890.10769494484702473No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC14890.10769494484702473No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA14130.1021980907111121No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14900.052.4849432
GTGGTAT15950.049.036911
GTATCAA53500.040.2033961
TATCAAC59250.036.1530842
GGTATCA28900.035.5945471
TCAACGC62250.034.6130874
ATCAACG62550.034.3111953
CAACGCA63500.034.0655825
AACGCAG64200.033.694156
ACGCAGA70750.030.5747647
CGCAGAG73150.029.6297268
GCAGAGT78950.027.6144879
CAGAGTA46600.025.44427310-11
TACCGTC1302.3723687E-522.8837097
AGAGTAC43150.021.4707610-11
GTACGGG53650.021.42739114-15
ACGGGAT8100.020.98682416-17
GAGTACG55700.020.71506912-13
TACGCCC1100.00548115519.3174174
AGTACGG48000.019.07997512-13
Produced by FastQC (version 0.11.5)