FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740334
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30740.17663276129754404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT28300.041.199991
TGGTATC29450.039.3896942
GTATCAA121800.027.4313181
AGAGTAC78050.025.79249411
GGTATCA58700.025.625181
TATCAAC134100.024.8034462
CAGAGTA82850.024.36456710
CGGGGTA3550.024.01463717
CCCTCGA600.002033974722.92048848
ATCAACG145850.022.7486863
TCAACGC146600.022.6510544
CAACGCA148150.022.4140725
AACGCAG150700.022.0725526
AGTACGG91450.022.01316813
GAGTACG91900.021.90537812
TACGGGG38350.020.50953515
GTCCGGA1108.255658E-619.99988655
ACGGGGC5850.019.74461216
ACGGGAT19100.019.43817916
ACGCAGA171350.019.380987
Produced by FastQC (version 0.11.5)