FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283466
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46060.3588719919343403No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36020.2806463124071849No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25090.195486284794455No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT15310.11928636987656861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12400.054.5008132
GTGGTAT13750.051.012491
GGTATCA29450.041.8608441
GTATCAA56900.040.1196631
TATCAAC64400.035.9038162
TCAACGC66600.034.522314
ATCAACG67150.034.2395483
CAACGCA67650.034.1121255
AACGCAG70650.032.9042436
ACGCAGA77850.029.8053287
CGCAGAG79000.029.2638658
GCAGAGT81450.028.3314369
CAGAGTA50200.025.98929610-11
AGACCGT1502.7912192E-622.6660336
GAGTACG58000.022.27437212-13
GTACGGG59000.021.86082614-15
AGAGTAC48250.021.44675310-11
ATATCGC1254.7847355E-420.3994316
ACGGGAT12300.019.69380616-17
AGTACGG54200.018.97474512-13
Produced by FastQC (version 0.11.5)