FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283466
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38780.3021505828748093No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8650.036.238972
GTGGTAT9750.033.5841371
GTATCAA47800.031.0279251
GGTATCA19850.028.0015931
TATCAAC53300.027.9614222
TCAACGC55100.026.6425194
ATCAACG56250.026.2933163
AGAGTAC53650.026.19221111
CAACGCA56750.026.0626755
CAGAGTA54500.025.83215510
AACGCAG57350.025.7920136
ACGCAGA58950.025.0929537
CGCAGAG59450.024.7894138
GCAGAGT61900.023.8545259
AGTACGG59850.023.29692813
GAGTACG62350.022.44927612
GTACGGG65200.020.96349314
TACGGGG24250.020.41341215
TACGGGA31750.020.00889415
ACGGGAA8100.019.69236216
Produced by FastQC (version 0.11.5)