FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816568
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73070.40224203002585096No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT56250.3096498452026018No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41760.22988404507841162No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT31760.17483518370906018No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28000.154136811834184No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT22500.12385993808104072No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18740.1031615662061646No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT30100.056.497891
TGGTATC30350.055.45082
GTATCAA109400.039.483451
TATCAAC121350.035.9668162
GGTATCA56800.035.4039341
TCAACGC130400.033.4054764
ATCAACG130800.033.3033183
CAACGCA133350.032.666475
AACGCAG136050.032.049426
GTCGAGG21500.030.6399828
ACGCAGA154950.028.1401967
TCGAGGT21750.028.1383429
AGTCGAG28300.027.931737
CGCAGAG155850.027.9246948
GCAGAGT163000.026.725859
GACAGGT9500.026.4038411
CAGAGTA82350.024.48882110-11
AGAGTAC79750.022.06301710-11
GAGTACG93650.021.5112612-13
GTACGGG101150.019.62159514-15
Produced by FastQC (version 0.11.5)