FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816568
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37240.20500195973946472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT35400.042.721541
TGGTATC36650.041.2462842
GTATCAA133600.025.7476921
TAACGGC651.0880368E-425.38896436
AGAGTAC84500.024.21516411
GGTATCA69800.023.67591
CAGAGTA88100.023.47476210
TATCAAC146200.023.386712
ATCAACG156700.021.7488783
TCAACGC157000.021.6723084
CAACGCA159350.021.3699465
AACGCAG162350.021.1443486
AGTACGG96450.020.98798413
GCCGTAT804.43851E-420.62853239
GAGTACG99300.020.44044312
TACGGGG41700.018.86362315
ACGCAGA184000.018.6440687
GTACGGG107950.018.5482914
TACGGGA51550.018.51381515
ACGGGGA17700.018.49135816
Produced by FastQC (version 0.11.5)