FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814449
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21110.2591936388896051No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20100.24679261684893714No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12780.15691590265320482No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA11450.1405858439263846No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT10430.12806203948927433No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA9360.11492432306995282No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC9200.1129598047268767No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT9160.11246867514110767No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9070.11136363357312735No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT9000.11050415679803155No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT8190.10055878268620871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58550.036.5966721
TGGTATC19350.036.4567872
GTGGTAT20100.034.9000361
AACGCAG66050.033.006226
TATCAAC68050.032.161062
TCAACGC70900.030.868274
CAACGCA71500.030.668675
ATCAACG71550.030.6472383
GCCGCTG853.491673E-529.99747
CGGGCCG853.491673E-529.99744
ACGCAGA72450.029.9732487
CGCAGAG72700.029.8701768
GACAGGT3600.029.524021
GGCCGCT905.1563504E-528.3308776
TACGGAG1203.9902443E-728.3308774
ACAGGTA3950.027.9842851
GCAGAGT79300.027.4377239
TTGTACG1106.4975957E-627.0431123
CGCTGTG957.4516865E-526.8397799
GGTATCA35450.026.7440151
Produced by FastQC (version 0.11.5)