FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814449
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA12580.15446025472435965No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12470.15310964836349483No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT8660.1063295553189948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18300.031.0846792
CGGGAGC952.4901965E-928.95189717
GTGGTAT19750.028.809591
TTCGCCG400.007865827.50430114
GGGCCGC400.00787051927.50092118
GCTTGTA3750.027.1209091
GTATCAA61250.025.535241
CCTTCGC1502.3646862E-1123.82973920
AGAGTAC47600.023.63289611
CGCGGCC1052.0937841E-723.57511711
CAGAGTA48000.023.43451910
TAGGCCA1453.947207E-1022.7426135
CTACGCA1705.456968E-1222.65199548
TATCAAC69050.022.6451762
AACGCAG70250.022.1045956
CTTCGCC1658.367351E-1121.66872621
ATCGGTA1658.367351E-1121.66872628
TCAACGC72650.021.3339184
CGGGCTC650.003249875321.1415636
ATCAACG73750.021.090243
Produced by FastQC (version 0.11.5)