FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758317
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47550.270429052326742No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38910.2212911551216305No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27440.15605832167919664No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT20810.11835181028221874No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18260.10384930589876569No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT21300.047.911111
TGGTATC22050.047.027422
GTATCAA77200.040.042551
TATCAAC86400.036.1529272
GGTATCA40150.034.5252341
TCAACGC90550.034.449074
ATCAACG90800.034.3074153
CAACGCA91850.033.9614945
AACGCAG95850.032.544226
ACGCAGA107800.029.0154257
CGCAGAG108700.028.6969958
GCAGAGT114850.027.1981019
GTCGAGG14450.026.1753238
CAGAGTA62550.024.32416510-11
TCGAGGT14950.024.1635079
AGTCGAG19900.022.4236417
GACAGGT11050.021.549161
AGAGTAC59750.021.0540710-11
GAGTACG73600.020.49900612-13
GTACGGG79700.018.69011514-15
Produced by FastQC (version 0.11.5)