FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758317
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43640.24819187893878064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT24950.041.4405751
TGGTATC25950.040.1478272
GTATCAA90250.025.4113241
CGGGCTA651.0886449E-425.3868117
AGAGTAC57200.024.42449211
GGTATCA47850.023.2071883
CAGAGTA60650.023.1705110
TATCAAC98800.023.0095832
TCAACGC105750.021.2362174
ATCAACG106150.021.2073783
CAACGCA107750.020.842045
AACGCAG109800.020.5780856
AGTACGG67950.020.3586413
GAGTACG70600.019.67237912
ACGGGAT12350.019.37414416
GACAGGT6900.018.7308371
TACGACT750.0074328518.325014
ACGCAGA125650.017.9833037
CGCAGAG126400.017.8351358
GTACGGG78650.017.48402814
Produced by FastQC (version 0.11.5)