FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513508
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16850.32813510208214863No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15580.303403257592871No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9650.18792307033191302No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT8100.15773853571901508No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC7160.1394330760182899No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA6800.1324224744307781No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6600.12852769577104933No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT6120.1191802269877003No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA5780.11255910326616139No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC5720.11139066966824275No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC5720.11139066966824275No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA5460.10632745741059536No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT5390.1049642848796903No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT5330.10379585128177166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGT1450.046.902571
ACAGCGG450.001662732537.775272
TGGTATC13250.036.563612
GCTTGTA2950.036.0215721
GTATCAA40650.034.506271
GCTTGTC1254.4383341E-1034.004361
GTGGTAT14750.033.4280171
GGCACCG2350.032.5541959
AGAGGCA2550.031.6645646
AACGCAG45350.030.9240766
ATACGGG1101.8566061E-730.9130571
TCCTGCG550.00444718630.9070368
GCTTGCC853.4840683E-530.003851
GCTTAGG853.4840683E-530.003851
CTTGAGT1701.2732926E-1129.9980092
TATCAAC47450.029.645032
TCAACGC48550.028.7982944
ATCAACG48900.028.7659823
TATTCCG600.006804337728.3314515
CAACGCA49450.028.188225
Produced by FastQC (version 0.11.5)