FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513508
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7870.153259540260327No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA7030.13690146988946617No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC6110.11898548805471384No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG5590.10885906353941906No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT5430.10574324061163605No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTT5340.10399059021475808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG553.4959437E-529.9888973
TGGTATC15500.029.7954222
GTGGTAT15950.028.9745481
GACAGGT1550.028.3959481
GCTTCTC705.685064E-627.5085771
GGTACCC2200.027.50321446
GGCCGCG2150.025.58687840
CAGACGC651.0855116E-425.3925321
GCGCCCC651.08767024E-425.38510911
AGACGCA651.09055414E-425.3752212
GTATCAA53150.025.257171
GCACCGC2300.025.10918410
CGCTAGT1101.1874363E-825.00048614
TGCTATC1101.1899829E-824.99561530
TTACGCA550.001228277924.9907473
GACACAT2450.024.69435926
CGGGACC901.4442139E-624.4449217
AGAGTAC36500.024.26074611
ATTAGAG2850.024.1138783
AACGGCC2400.024.06765737
Produced by FastQC (version 0.11.5)