FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938889
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31470.3351833922859891No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22080.23517156980218107No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA14960.1593372592500285No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14850.15816566175554297No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC11440.12184613942649238No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT10960.11673371399601018No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT10290.10959762016596211No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG10000.10650886313504579No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT9700.10331359724099441No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10650.054.675062
GTGGTAT11450.051.2316321
GTATCAA43000.040.8268781
GGTATCA22300.040.4106251
TATCAAC49950.035.3977932
TCAACGC50600.034.7713784
ATCAACG51250.034.3303763
CAACGCA52150.033.7379045
AACGCAG53350.032.979046
ACGCAGA58000.030.3350287
CGCAGAG58450.030.174198
GCAGAGT62250.028.2639649
CAGAGTA40300.025.7310110-11
AGAGTAC38900.022.56017910-11
GAGTACG47600.021.69558712-13
ACGGAAT1000.003155946721.2491745
GTACGGG51900.019.69335714-15
AGTACGG45250.019.30035412-13
CCGTCTT11050.018.84642684-85
CCGCACA1150.007083410418.4775434
Produced by FastQC (version 0.11.5)