FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268047
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33060.26071588829120684No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32250.2543281124437817No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21670.17089271927617825No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT17600.13879611717862192No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22600.044.756142
GTGGTAT22350.044.1332441
GTATCAA72500.039.1736341
TATCAAC82350.034.835682
ATCAACG87650.032.777733
TCAACGC87800.032.721734
AACGCAG87850.032.7031066
GGTATCA38650.032.010971
CAACGCA91000.031.664485
ACGCAGA98650.029.1659187
CGCAGAG101150.028.4870788
GTCGAGG10800.028.3330158
GCAGAGT108650.026.6009739
AGTCGAG16650.024.248987
TCGAGGT12150.024.137449
CAGAGTA62900.022.4677410-11
GACAGGT4900.021.6919841
GTATTAG5350.021.456821
AGAGTAC60150.020.5978210-11
GACGGTC1050.00418715720.2378717
Produced by FastQC (version 0.11.5)