FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268047
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19170.15117736172239674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT26050.036.9458471
TGGTATC27050.035.4615632
GTATCAA86000.024.9402851
GACAGGT3650.023.3546891
TATCAAC93850.022.7847922
AGAGTAC61550.022.34432211
GGTATCA45550.021.6726171
CAGAGTA64700.021.38399910
GCTTGTA3750.021.2653241
ATCAACG101350.021.097863
AACGCAG101450.021.0778966
TCAACGC101300.021.0268784
CAACGCA102950.020.7699665
ACGCAGA114450.018.6611847
CGCAGAG115800.018.3508558
AGTACGG77650.017.53501313
GCAGAGT121150.017.5226259
GAGTACG79300.017.2735412
CGTAAGC1000.001990310816.49765629
ACGGGCT4900.016.27962116
Produced by FastQC (version 0.11.5)