FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868426
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18250.21015031793152208No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16040.18470197806145833No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.1271265484911783No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT9440.10870241102868869No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12600.046.2208981
TGGTATC13050.044.939882
GTATCAA39850.036.162611
GGTATCA24600.031.2774871
TATCAAC46100.031.0665022
TCAACGC47600.030.1767944
ATCAACG48200.029.7129783
AACGCAG48450.029.5596626
CAACGCA48800.029.4347425
ACGCAGA55200.025.945037
CGCAGAG55250.025.7677148
TCGACGT5400.025.1851529
GTCGACG5700.023.8582428
GTCGAGG6650.023.6452248
GCAGAGT60750.023.5061429
CAGAGTA35500.022.50701110-11
ACGTACG704.2049287E-521.24874720-21
CGTCAGA2006.672235E-821.2487475
TCGAGGT7750.020.2902959
TACGGGG20000.019.7613352
Produced by FastQC (version 0.11.5)