FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868426
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13640.1570657718677239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15100.035.8518642
GTGGTAT15200.035.8029751
GTATCAA51650.026.8199351
AGAGTAC38050.025.37320111
CAGAGTA39150.024.6602910
TATCAAC57900.023.7308852
GCTACGG600.002044284522.8989853
TCAACGC59650.022.8951474
ATCAACG60000.022.8073883
AACGCAG60200.022.6416286
CAACGCA60900.022.425215
GTATAGG2350.021.0524251
ACGCAGA65700.020.750997
GGTATCA27100.020.6851353
CGCAGAG66050.020.6065378
AGTACGG47350.020.09920713
GAGTACG48350.019.79728112
GCAGAGT69550.019.6497449
AAGGCGC1151.20840195E-519.13440310
CAATCGT1602.997149E-818.90799355
Produced by FastQC (version 0.11.5)