FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555959
Sequences flagged as poor quality0
Sequence length91
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91290.5867121177357502No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT62430.40123165199083005No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55670.35778577713165965No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT35810.230147452471434No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34550.22204955271957683No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29920.19229298458378402No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT24400.15681647138517146No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21860.1404921337901577No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21060.13535061013818486No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18080.11619843453458606No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16270.10456573727199753No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16130.10366597063290227No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT27350.056.4255751
TGGTATC27700.055.692932
GTATCAA109200.039.827191
GGTATCA55850.036.690241
TATCAAC122600.035.7735822
TCAACGC132400.033.0935864
ATCAACG132700.033.0507973
CAACGCA134650.032.6984065
GACAGGC2750.032.4648441
AACGCAG139700.031.5468126
GCGTCAG2450.029.4991531
GTCGAGG23300.028.8196248
CGCAGAG159050.027.629568
ACGCAGA161300.027.3495467
TCGAGGT23900.026.6735299
GCAGAGT165450.026.612169
CGGGACG1009.458745E-1125.50194416-17
TACCGCG1001.05618536E-425.4998939
GACAGGT8800.025.1216051
AGTCGAG34450.024.6733387
Produced by FastQC (version 0.11.5)