FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105024
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41570.3761909243600139No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31760.2874145719911966No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21530.19483739719680296No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT14390.13022341596200626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10900.048.345872
GTGGTAT11750.045.5780561
GTATCAA45450.043.298241
TATCAAC50050.039.3134462
ATCAACG52450.037.4318283
TCAACGC52900.037.1117254
CAACGCA54350.036.1216285
GGTATCA23250.035.830781
AACGCAG55650.035.2778136
ACGCAGA61350.031.9987247
CGCAGAG62200.031.5614418
GCAGAGT66500.029.5845229
CAGAGTA42800.024.67106410-11
GTATAGA2403.6379788E-1123.0226481
GAGTACG45900.022.91225212-13
AGAGTAC39500.022.2678610-11
ACGGGAT8500.021.74580816-17
GTACGGG48250.021.57615314-15
AGTACGG43050.020.28354812-13
TACGGGG18700.019.88253214-15
Produced by FastQC (version 0.11.5)