FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131601
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117791.0409145979899277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8100.034.6328282
GTGGTAT8050.033.4991231
GTATCAA41850.029.522571
GGTATCA19400.027.800821
TATCAAC47300.026.048922
AGAGTAC46450.024.98174711
ATCAACG49400.024.7679883
CAACGCA49600.024.613775
TCAACGC49600.024.5018144
CAGAGTA48250.024.33473610
AACGCAG50400.024.3311126
ACGCAGA52300.023.4471937
CGCAGAG52400.023.2975038
AGTACGG51050.022.35661313
GCAGAGT55200.022.2671439
GAGTACG53550.021.41370412
TACGGGG21500.019.95444915
GTACGGG56800.019.75447514
CGGGATA2105.456968E-1219.64380317
TATACCG700.004979687319.6403335
Produced by FastQC (version 0.11.5)