FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106607
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50280.4543618466176339No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40930.3698693393408861No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29900.2701952906497067No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT22930.20720996704340386No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA21130.1909440298136556No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18160.16410523338457106No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC16810.15190578046225986No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.15154431519048767No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT15750.142326950760297No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG14610.1320251905147898No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA14180.12813943884323883No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT13980.12633211248437792No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA13710.12389222189991568No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC13000.11747621332595944No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11860.10717445308045222No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA11820.10681298780868004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20800.054.3500062
GTGGTAT21600.051.971711
GTATCAA80350.040.1143881
TATCAAC90700.035.658142
GGTATCA42350.034.4395561
TCAACGC98150.033.0381364
ATCAACG98750.032.794363
AACGCAG100500.032.3924676
CAACGCA100950.032.290175
GTCGAGG15850.028.6916458
ACGCAGA113900.028.6201957
CGCAGAG113250.028.5968198
GCAGAGT117950.027.4945859
TCGAGGT16200.026.7612089
CAGAGTA60600.024.1271710-11
AAGTACG2152.3101165E-1023.7333831
CGGGTCG900.001711199123.6106976
GCAGCGT2352.7284841E-1123.5229821
AGTCGAG23900.023.2956247
GACAGGT5150.022.293251
Produced by FastQC (version 0.11.5)