FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106607
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22920.2071196007254608No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT18450.16672585660491937No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT16310.14738746456510757No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC15480.13988706017583477No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC12970.1172051143721303No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA12260.11078910579817407No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT11780.10645152253690787No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA11770.10636115621896483No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG11450.10346943404478735No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT11370.10274650350124298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT29500.040.277151
TGGTATC30200.039.0491642
GTATCAA104000.025.388371
AGAGTAC65650.023.7523211
CAGAGTA67100.023.28002710
TATCAAC113250.023.2292462
AGTTCGC952.320161E-623.1529458
CGCCGTC600.002034265322.91902235
CGGGCGA600.002034800122.91798617
GACAGGT3700.022.3005961
GGTATCA55950.022.2064973
GTCTTAC1505.966285E-1022.0032581
TCGTAGA752.8802376E-421.9913232
ATCAACG122650.021.4703793
TCAACGC123550.021.2917294
CGACACG650.003237744621.1560238
AACGCAG125450.021.055956
CAACGCA126450.020.8686395
ACTATCG2053.6379788E-1220.1231111
AGTACGG77350.019.87509313
Produced by FastQC (version 0.11.5)