FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672192
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43600.6486242026087784No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28210.4196717604493954No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18490.27507021803294296No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT14770.21972888698467108No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC7720.11484813862705893No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6840.1017566409597258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC11850.053.7985042
GTGGTAT12050.052.9173971
GTATCAA41150.038.9462621
TATCAAC45400.035.011692
TCAACGC46800.034.055144
ATCAACG46950.033.946343
AACGCAG47400.033.713736
CAACGCA47500.033.6427545
GGTATCA22050.032.1960141
ACGCAGA53550.029.9983467
CGCAGAG54750.029.2632278
GGTACGG905.1513063E-528.333883
CTGCGAA755.904982E-428.331779
GCAGAGT57850.027.7685649
GTATACT1159.165649E-625.8700624
CAGAGTA31900.025.17886210-11
AGAGTAC30900.022.34909210-11
TATTAGG1152.721694E-422.174342
GAGTACG36850.021.73896412-13
GATACCG1604.877573E-621.2504085
Produced by FastQC (version 0.11.5)