FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753271
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38660.5132283069439816No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT36260.4813672635744639No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29350.38963400953972743No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19780.2625880991037754No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC8310.11031886266695519No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.10633623224576548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10750.055.3501781
TGGTATC10750.054.1605072
GTATCAA36750.039.7832761
GGTATCA20250.035.8897551
TATCAAC40800.035.727662
ATCAACG44400.032.8308223
TCAACGC44800.032.7274134
CAACGCA45150.032.567845
AACGCAG47500.031.0460596
CGCAGAG51650.028.716128
ACGCAGA52800.028.1711627
GCAGAGT54950.027.1462619
CAGAGTA31200.024.4500910-11
GAGTACG35000.021.7955112-13
AGAGTAC29650.021.4282710-11
GACAGGT3001.8189894E-1221.2505151
TAACCCG1000.003155435421.2491045
ACGGGAT7000.020.94554516-17
GTACGGG36700.020.67010914-15
ACGGGGA6650.020.1307316-17
Produced by FastQC (version 0.11.5)