FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766267
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15530.20267087059732442No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT12110.15803890810905338No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT10010.1306333171074834No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC8940.11666951597811208No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA7920.10335822892020667No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT7810.10192269796298156No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC7750.10113968107722242No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7000.031.4335332
GTATCAA30700.030.8312781
GTGGTAT7150.030.786151
TATCAAC34900.027.1891062
GGTATCA13800.025.7206571
AGAGTAC35550.025.59712411
TCAACGC37550.025.4018674
CAGTCGG651.08878005E-425.3836514
ATCAACG37450.025.3278563
AACGCAG37600.025.296636
CAGAGTA36150.025.17227610
CAACGCA37900.025.0963955
CGCAGAG39050.024.1445548
ACGCAGA39350.024.100197
GCAGAGT41000.022.9321659
GTAGACG600.002036135422.914326
AGTACGG39500.022.90419213
GAGTACG40450.022.43132412
ACTATCG1701.2551027E-1021.0231211
GTACTAT1207.545532E-720.6363411
Produced by FastQC (version 0.11.5)