FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203778
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35600.16154077225564462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCG458.854413E-636.6627228
CGACGAG2950.035.41991424
CTGGTCG3900.033.14121650
CAATGCG3250.032.15184419
TCAGCGC4300.030.05967944
CGTCTAC4300.029.42211355
GTCGTCA4450.028.42390324
CGTACCG606.320106E-527.5001610
GGCGTCG1203.6379788E-1127.4976628
CGTAACG705.714832E-627.49703823
CATTGCG4750.027.20759429
CAGCGCT4900.026.94015545
CGCCAGC4800.026.92479334
TGGTATC9500.026.6383842
CTGTGCG5200.026.4424619
TGGTCGT5100.025.88250251
TCGTCTA4800.025.7849154
AGGTCGT5050.025.59072122
ACCGGTC651.0893641E-425.38476413
AATGCGA4450.025.33549920
Produced by FastQC (version 0.11.5)