FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951396
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36070.3791270932398286No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34780.36556807049850953No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22560.23712523491795215No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT18410.19350512299820474No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT14440.15177696774003677No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12960.1362208796337172No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA12670.13317272723450593No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC10720.1126765300673957No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10310.10836707322713152No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT9700.10195544231844574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22250.047.598211
TGGTATC23700.046.623382
TATACCG450.001663094737.776895
GTATCAA77550.036.6366651
ATACGGG1254.4201443E-1034.0260281
TATCAAC87100.033.569822
GGTATCA43100.032.4668271
ATCAACG94800.031.0673083
AACGCAG94300.030.9616266
TCAACGC95400.030.8719164
GTCGAGG13700.030.4007458
CAACGCA96800.030.3815175
TCGAGGT14650.027.8491769
ACGCAGA108850.026.9010687
CGCAGAG109000.026.7080888
AAGTACG2159.094947E-1225.7173441
GCAGAGT115100.025.2557059
AGTCGAG20100.025.1610987
CAGAGTA60800.024.22087310-11
GACAGGT5200.022.9021341
Produced by FastQC (version 0.11.5)