FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951396
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15370.161552077158197No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT11060.11625022598371236No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT9790.10290142064923544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC26500.036.830122
GTGGTAT27550.036.0422331
GCACCGC801.5013939E-830.93764710
GTATCAA93650.026.4926171
AGAGTAC61200.024.80403911
CAGAGTA62750.024.103710
GACAGGT3100.023.9567661
TATCAAC104800.023.5840852
TTAAACG1750.023.5715422
TAACGGC952.3141329E-623.15922436
GTCTAGG1003.6074616E-622.0047341
CGGGGTC3750.022.00010517
GCGGTAC1003.6189995E-621.99663744
AACGCAG113250.021.7746936
TCAACGC113850.021.6840864
ATCAACG113800.021.6694533
GGTATCA48500.021.6530653
CAACGCA115700.021.3373645
ACTATCG1055.515938E-620.9524811
GGCACCG1055.51845E-620.951389
Produced by FastQC (version 0.11.5)