FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960617
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31510.32801834654185796No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28350.2951228221028776No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17390.18102948417527484No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT14840.1544840451501483No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11680.12158852071116792No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC11260.11721633075408827No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT11130.11586303386261121No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT11100.11555073457996266No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA10540.10972114797052311No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA10210.10628585586138908No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA9690.10087266829548092No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC9650.10045626925194953No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC9620.10014396996930097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC26900.037.444812
GTATCAA80600.034.774631
GTGGTAT28500.034.3237841
AACGCAG87000.032.5821386
CGGGTCC550.00444618230.9111759
TATCAAC92000.030.7667732
TCAACGC94600.029.9211754
ATCAACG95750.029.561813
AAGTACG2600.029.4449841
GACAGGT3650.029.1312641
CAACGCA97750.029.0004445
ACGCAGA98850.028.7192297
CGCAGAG99600.028.46038
GGTACGG1755.5479177E-1026.7146953
GCAGAGT107300.026.499929
GTCGAGG10950.026.3917248
GGTATCA47950.025.7229961
GCTTGTA6450.024.3979991
CAGAGTA65750.022.4634910-11
CAGGTAT3800.022.3850781
Produced by FastQC (version 0.11.5)