FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960617
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16380.17051540832610707No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT10510.10940884868787457No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT10270.10691045442668617No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA10160.10576535705697483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC28850.029.8159282
GTGGTAT29900.028.9677851
GTCCGGA1156.6393113E-1026.30365255
GACAGGT2650.025.9400041
GTATCAA91650.025.3512971
CCGATCG1201.0859367E-925.21160546
AGAGTAC66250.024.45477711
AGTCCGG1251.7425918E-924.2018854
CAGAGTA67450.024.14078710
GGCACCG2950.023.3044689
AACGCAG100650.022.6343466
TATCAAC102450.022.6133772
CTTGCGG2200.022.49940355
CCCTTGC2100.022.26479353
GCACCGC2950.021.44457810
TCAACGC105750.021.412834
ATCAACG106650.021.3094313
TTGTACG650.00325260821.138973
GGCCGCG3000.021.08716840
CAACGCA108850.020.8534935
Produced by FastQC (version 0.11.5)