FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409950
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30650.21738359516294903No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA16500.11702542643356147No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA15410.10929465583885954No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCA14100.10000354622504344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAA150.005784515854.9984749
CTATCGA257.865029E-443.9909743
CGCGGTA504.124031E-738.50166343
CGGTACC504.124031E-738.50166345
GTGGTAT11250.033.993721
TGGTATC11950.032.2201082
GTGTACG752.9338844E-729.34711
CGAGAGA904.7111826E-827.50607138
AACGGCC805.170841E-727.50606737
CCAACCG904.719186E-827.50118843
ACGCCGT507.035313E-427.49826223
ACGAGAG957.957533E-826.0583837
GGCGAGA858.800653E-725.88071830
CGAGAAC1001.3109457E-724.74843632
GTATCAA41050.024.7314571
GCGGTAC801.6080285E-524.06354144
GGTATCA21700.023.9628521
TTAACGG701.7977803E-423.57746735
CAGTCCG701.8012436E-423.5707749
CGGCAAC600.002036084722.9160312
Produced by FastQC (version 0.11.5)