FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161136
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31400.27042482534345674No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29880.25733419685549325No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC20170.17370919513304212No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18400.15846550274903198No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA18030.15527896818288298No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA18010.15510672307119924No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT16810.14477201637017542No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC16150.1390879276846123No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA15760.13572914800677957No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC15640.1346956773366772No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT15590.13426506455746784No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT15220.13107852999131886No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA13640.11747116616830414No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT13640.11747116616830414No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA13150.11325116093205276No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC12730.10963401358669442No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA12700.10937564591916882No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA12430.10705033691143845No Hit
GTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGA12410.10687809179975472No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG12240.10541400835044303No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.10162461589340094No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT11660.10041890011161483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC24300.044.421012
GTGGTAT26100.041.369961
GTATCAA80550.037.2062151
AACGCAG92650.032.3832366
TCAACGC95300.031.6165394
ATCAACG96600.031.2790433
TATCAAC99150.030.6031742
CAACGCA99950.030.1881525
GGCACCG8000.029.2181289
ACGCAGA103050.029.115067
CGCAGAG103950.028.82218
TTGTACG1951.8189894E-1228.331513
ACAGGAG5150.028.0649071
GACAGGT4850.028.0478881
GCAGAGT110850.026.9525179
GGTATCA45700.026.882741
AGACAGG4750.026.848471
ATTAGAG8650.026.5300833
AGAGGCA8650.026.5300836
GTCGAGG10150.025.540238
Produced by FastQC (version 0.11.5)