FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111977
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT53260.4789667412185684No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50280.452167625769238No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38250.3439819348781495No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25340.22788241123692307No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT12380.11133323800762067No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA11210.10081143764664197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13200.054.113041
TGGTATC13900.051.065962
GTATCAA50850.039.7999571
TATCAAC55650.036.4320032
GGTATCA26600.035.1647571
TCAACGC58850.034.4463464
ATCAACG59600.034.1554873
CAACGCA59850.034.0128175
AACGCAG62650.032.764036
ACGCAGA69900.029.1807377
CGCAGAG70550.028.9118848
GCAGAGT73200.027.8652119
CAGAGTA43150.025.06335310-11
AGAGTAC41250.022.1486210-11
GAGTACG49100.022.06941812-13
GTACGGA1553.6952442E-621.944461
GAGTTAG2251.0102667E-820.786281
TCTAGAC1909.582345E-720.1307583
GTATAGA2551.9535946E-920.0081831
GTACGGG54300.019.87769714-15
Produced by FastQC (version 0.11.5)