FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417100
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT82080.5792110648507516No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53040.3742855126667137No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35960.2537576741232094No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA22850.16124479570954767No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC17100.12066897184390658No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT16940.11953990544068874No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG15050.10620280855267801No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC23750.047.237892
GTGGTAT23700.046.9938551
GTATCAA69150.038.8519631
TATCAAC81250.033.6831472
GGTATCA39150.033.4431421
TCAACGC85200.032.0716674
ATCAACG85400.031.996563
CAACGCA86300.031.7121165
AACGCAG88100.031.1124326
CGCAGAG99050.027.6719688
ACGCAGA99550.027.4893287
GCAGAGT104650.026.2318029
CAGAGTA53400.025.3863110-11
GAGTACG58050.023.09696812-13
AGAGTAC51800.022.47868710-11
GACAGGT6350.022.091661
AGTACGG57300.020.0989112-13
GTGTAGA4350.019.5446911
ACGGGTC4550.019.14630516-17
GTACGGG71000.018.85428414-15
Produced by FastQC (version 0.11.5)