FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417100
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21640.15270623103521275No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT19390.1368287347399619No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT15210.10733187495589586No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC14390.10154540963940442No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT15550.032.1996151
TGGTATC16250.031.3038752
GTATCAA59650.028.226091
CTAGCGT507.0508244E-427.4879154
TATCAAC69500.024.4898192
AGAGTAC64000.024.23056611
GGTATCA30050.024.1695841
CAGAGTA65350.023.81331810
TCAACGC71250.023.5720924
AACGCAG71650.023.4821766
CAACGCA72150.023.4693745
ATCAACG71950.023.4208223
ACGCAGA74650.022.5753127
CGCAGAG75150.022.425118
CGATCGT752.8734267E-421.9996547
ACGGGGG5800.021.3373716
AGTACGG70200.021.31170313
GAGTACG71550.021.09954512
GCAGAGT79900.021.0941879
ACGGGAT8900.019.7763616
Produced by FastQC (version 0.11.5)