FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053018
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG107571.0215399926686912No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC12020.11414809623387255No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT11140.10579116406367221No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC10550.1001882209041061No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC9050.035.5492942
GTGGTAT9500.034.46721
GTATCAA48400.032.1776051
TATCAAC54900.028.4491752
CCGCGCA400.00786716427.50406548
AGAGTAC53950.026.8578711
GGTATCA20650.026.5164661
TCAACGC58300.026.4957244
CAGAGTA55050.026.46852310
ATCAACG58750.026.38763
CAACGCA59700.026.0112675
AACGCAG59950.025.9052586
ACGCAGA61400.025.2487227
CGCAGAG61300.025.155398
GCAGAGT64550.024.0188799
AGTACGG60250.023.18574113
GAGTACG60900.022.98233612
GTATAGC600.002028617322.9298571
ACGGGGG6050.022.27390316
TACGGGG23400.021.742615
Produced by FastQC (version 0.11.5)