FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200707
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33600.15267820750331598No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC24960.11341809700246329No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA24180.10987378147113633No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTT22700.10314866995015694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC55350.029.4980852
GTGGTAT56900.029.186231
TCGGCCG400.00786946327.50372923
GTATCAA184650.026.1176221
AGAGTAC132250.025.24555811
CAGAGTA133700.025.19746210
AACGCAG201650.023.675926
TATCAAC204650.023.4776552
TCAACGC212000.022.6092594
ATCAACG213500.022.50193
CAACGCA216000.022.2032935
GCACCGC7150.021.1547910
GACAGGC3000.021.0794811
ACGCAGA227600.020.997
GCTTGTA9200.020.9200921
CGCAGAG228150.020.9061058
CTTGCGG3450.019.9293355
GCAGAGT240000.019.8898419
TTGTACG2700.019.3404033
AGTACGG173750.019.02660813
Produced by FastQC (version 0.11.5)