FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860598
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105640.5677744467101438No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT74860.4023437625967565No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59460.3195746743788825No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT42940.23078601610879942No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42610.22901239278984498No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT28940.15554139045618667No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28390.1525853515912626No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26100.14027748068094237No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24460.13146311024735058No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA19200.10319262946644037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC30650.061.5652122
GTGGTAT33700.056.139011
GTATCAA133950.041.8001371
TATCAAC145800.038.6808782
GGTATCA68200.037.1532251
ATCAACG161600.034.8726583
TCAACGC162300.034.6436964
CAACGCA166250.033.8717085
AACGCAG170550.033.0426376
CGCAGAG189750.029.721598
ACGCAGA191400.029.4875747
GCAGAGT196750.028.6857519
GTCGAGG26100.027.1931088
CAGAGTA90150.025.29221510-11
TCGAGGT27200.024.6871579
AGAGTAC86850.022.53422710-11
AGTCGAG40250.022.384787
GACAGGT11250.022.2963681
GAGTACG104550.021.6460412-13
GTACGGG115050.019.41194214-15
Produced by FastQC (version 0.11.5)