FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402558
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50601.2569617297383235No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35190.874159748408925No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT26200.6508378916826891No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23070.5730851206534213No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC15690.38975750078249594No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.21810521713641265No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.18357603127996464No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA6920.17190069505512248No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.1540150736043005No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT5560.13811674342579205No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT5070.12594458438287154No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC4950.1229636474744012No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA4770.1184922421116957No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC4510.11203354547667664No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT4500.11178513406763745No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG4470.11103989984051986No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA4040.10035820925183453No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4030.10010979784279533No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAC150.001029327484.994665
TGGTATC11350.056.5523452
GTGGTAT12100.052.7022821
GTATCAA44550.040.843181
TATCAAC48900.037.4660572
GGTATCA24450.035.1233561
TCAACGC52900.034.785154
CAACGCA53050.034.76695
ATCAACG53150.034.7014853
AACGCAG53750.034.393196
GACAGGT1501.8189894E-1234.0105361
ACAGGTA1900.031.3254951
GACGTGT550.004445977530.9071466
ACGCAGA60450.030.6515067
CGCAGAG60800.030.4051638
CGGGTCG503.094403E-629.7481316-17
GCAGAGT62600.029.5308919
GTCGTGG1054.503965E-628.3421131
TCGTAAC600.00680249628.3315533
CCCGCAC600.00680249628.3315538
Produced by FastQC (version 0.11.5)